cGMP Direct Immunoassay Kit (Colorimetric)

  • Catalog number
    K372-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Highly Sensitive Assay
  • Summary
    • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
  • Sample Type
    Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
  • Features Benefits
    • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
    cGMP   Direct   Immunoassay   Kit  
  • Short name
    cGMP Direct Immunoassay Kit (Colorimetric)
  • Technique
    colorimetric, immunoassay, colorimetry
  • Alternative name
    cGMP Direct Immunoassay reagent (colorimetry)
  • Alternative technique
    assays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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