Mouse IgG1 Kappa Light Chain Isotype Control FITC

• Catalog number
  61R-I170aFT
• Price
  Please ask
• Size
  100 tests

• Category
  Biological Reagents
• Antibody Subtype
  Isotype Controls
• Area of research
  Immunology
• Type of Immunogen
   
• Raised in
  Mouse
• Cross Reactivity
   
• Isotypes
  IgG1 kappa
• Clone
  M12637
• Form Buffer
  Affinity purified and Provided in PBS, pH 7.2, with 0.2% BSA and 0.09% NaN3.
• Storage
  Store at 4 deg C. DO NOT FREEZE. Keep away from light.
• Shipping conditions
  Blue Ice
• Tested for
  ICC; IF; IHC; IP; FC; WB
• Usage Recommendations
  FC: 20 ul per 106 cells in 100 ul volume
• Assay Information
  This immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
• URL
• More info
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  IgG1   Kappa   Light   Chain  
• Short name
  Mouse IgG1 Kappa Light Chain Isotype Control FITC
• Technique
  Control, Isotype, light, Mouse, FITC, controls, Fluorescein, Isotypes and controls, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  FITC Conjugated Mouse IgG1 Kappa Light Chain Isotype Control antibody
• Alternative technique
  control, controls, murine, fluorescine
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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