Description Rat IgG2a was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Test A high affinity purification column was use to purify IgG2a Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.
Additional description 1 of protein A or G purified by Elabscience will give more specificity than crude serum.
Properties If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
Additional conjugation Biotinylated
About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name Rattus norvegicus
French translation anticorps
Short name IgG2a Isotype Antibody -Conjugated
Technique affinity, Antibody, purified, Isotype
Alternative name protein enriched Rat IgG2a isoform (Antibody to) biotinilated-coupled