anti-Fas Ligand Antibody
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Catalog numberXP-5130
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PricePlease ask
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Size0.1 mg
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Gene infoFas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
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Gene namesAPTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL
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Antibodie s labelunlabelled serum
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PurityAnti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.
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Ab raised inGoat
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TypePolyclonal
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AntigenProduced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL (human FAS Ligand).
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Immuno applicationELISA, Western Blot, NELISAut
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Antibody adviceNeutralization: To yield one-half maximal inhibition [ND50] of the biological activity of FasL/Apo1L (10 ng/mL), a concentration of 0.08 - 0.1 ug/mL of this antibody is required. ELISA:To detect hFasL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFasL. Western Blot:To detect hFasL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep atFas Ligand antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works withHuman
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Omim nr1345957
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Ncbi nrP48023
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Vial with antibodyLyophilized
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NCBI GENE nr356
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GENE symbolFASLG
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Antigene nameFas ligand (TNF superfamily, member 6)
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SourceHomo sapiens
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Protein nrP48023
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolFASLG
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Short nameanti-Fas Ligand Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toFas Ligand (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetFas (TNF receptor superfamily, member 6), ALPS1A and APO-1 and APT1 and CD95 and FAS1 and FASTM and TNFRSF6, FAS and IDBG-81669 and ENSG00000026103 and 355, identical protein binding, nuclei, Fas and IDBG-159577 and ENSMUSG00000024778 and 14102, FAS and IDBG-637356 and ENSBTAG00000010785 and 282488
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Gene info
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Identity
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Gene
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Long gene nameFas ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 6
- Fas ligand (TNF superfamily, member 6)
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-12-09
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
- Death inducing signaling complex
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data