His Tag Antibody
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Catalog numberF52067-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS pH 7.4 with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenHis Tag
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivity; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, ELISA
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Recommended dilutionsWestern blot: 1:1000
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NotesTitration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis anti-His Tag antibodyis to be used only for research purposes and not for diagnostics..
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PurityPurified
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DescriptionEpitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.
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ImmunogenHHHHHH peptide was used as the immunogen for the anti-His Tag antibody.
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StorageAliquot the anti-His Tag antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional conjugationhistidine
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French translationanticorps
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Gene target
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Short nameAnti-His Tag
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TechniqueAntibody, antibodies against human proteins, antibodies for, Tags
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IsotypeRabbit IgG
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LabelHis
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Alternative nameAntibodies to His Tag
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data