Akt1, Human, phosphorylated, active, Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant

  • Catalog number
    MBS635664
  • Price
    Please ask
  • Size
    0.1 mL
  • Products_type
    Recombinant Protein
  • Products_short_name
    [Akt1, active, Western Blot Positive Control]
  • Specificity
    This preparation was active (Specific Activity: 300-1800u/mg). AKT1 was activated with MAPKAP kinase 2 and PDK1 and repurified.
  • Purity
    Highly PurifiedHighly purified (90%).
  • Form
    Supplied in SDS-PAGE sample buffer (reduced). This preparation of AKT1 is phosphorylated but not active in sample buffer.
  • Storage_stability
    May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6-12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Tested_application
    Western Blot (WB)
  • Description
    The Akt1, , phosphorylated, active, Blot Positive Control (v-akt Mouse Thymoma Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant is a α- or alpha protein sometimes glycoprotein present in blood. Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts. Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+ 3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.
  • Test
    Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Latin name
    Mus musculus
  • Group
    positif, recombinants
  • Source
    Recombinants or rec. proteins
  • Gene target
  • Gene symbol
    AKT1, AKT3, RAC1, CEBPA, SRPRA, SMC4, CBX5, AGPAT1, DRAP1, MYB
  • Short name
    Akt1, , phosphorylated, active, Blot Positive Control (v-akt Mouse Thymoma Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant
  • Technique
    Western Blot, Recombinant, Positive, Control, Mouse, Blot, western Blots, controls, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    mouse
  • Species
    Mouse, Humans, Mouses
  • Alternative name
    v-akt murine thymoma viral oncogene homolog 1, H. sapiens, phosphorylated, functionnal, Western Blot Positive reference (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein phosphorylation catalyst B, RAC, RAC-a, RAC-a serine/threonine-protein phosphorylation catalyst, RAC-PK-a) Rec.
  • Alternative technique
    rec, controls, murine, western
  • Alternative to gene target
    v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
  • Tissue
    control
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    CCAAT enhancer binding protein alpha
  • Synonyms gene
  • Synonyms gene name
    • CCAAT/enhancer binding protein (C/EBP), alpha
    • CCAAT/enhancer binding protein alpha
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1991-11-27
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Basic leucine zipper proteins
    • CCAAT/enhancer binding proteins
  • VEGA ID
  • Locus Specific Databases
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    structural maintenance of chromosomes 4
  • Synonyms gene
  • Synonyms gene name
    • SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
    • SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-02-22
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Structural maintenance of chromosomes proteins
    • Condensin I subunits
    • Condensin II subunits
    • MicroRNA protein coding host genes
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
    MYB
  • Long gene name
    MYB proto-oncogene, transcription factor
  • Synonyms gene name
    • v-myb avian myeloblastosis viral oncogene homolog
  • Synonyms
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Myb/SANT domain containing
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Process of growing viruses in live animals, plants, or cultured cells.
  • Tree numbers
    • E01.370.225.875.970
    • E05.200.875.970
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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