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Also known as
Integrin alphaV, beta3
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Other names
integrin beta-3; N/A; integrin beta-3; beta3 integrin; integrin beta 3; glycoprotein GPIIIa; N/A; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ITGB3; N/A; CD61; N/A
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Gene name synonims
ITGB3; N/A; CD61; N/A
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Other gene names
ITGB3; N/A; CD61; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
2Q880
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Avian, Bovine, Canine, Chicken, Human (Homo sapiens), Monkey, Porcine, Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: bovine, chick, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein A affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS pH 7.6, 0.1% sodium azide, 15mg/ml BSA, before the addition of glycerol to 40%. Labeled with FITC.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applications
Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
