Caspase-8 (phospho-T341) polyclonal antibody
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Catalog number
AP0237-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
Caspase-8; Caspase8; Caspase 8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Caspase-8 subunit p18; Caspase-8 subunit p10; CASP8; MCH5
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Immunogen
Synthetic phosphopeptide derived from human Caspase-8 around the phosphorylation site of Threonine 341.
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Swiss Prot
Q14790
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Reactivity
Human,Mouse,Rat
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Applications
WB
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Application_all
WB: 1:500~1:1000_x000D_
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
p-Caspase 8 (T341) polyclonal antibody detects endogenous levels of Caspase 8 protein only when phosphorylated at Thr341.
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Usage
Western blot (WB) analysis of p-Caspase-8 (T341) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug) Lane3:HEK293T treated with UV for 6h whole cell lysate(40ug) Lane4:HEK293T treated with UV for 24h whole cell lysate(40ug)
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BiowMW
~ 38, 55 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Short name
Caspase-8 (phospho-T341) polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
caspase-8 (phosphorilated-T341) polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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