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Alternative name 1
CD8b, Lyt-3, T-Cell, Cytotoxic/Suppressor, Clone: YTS 156.7, Rat Mab anti-Mouse, FITC; frozen, IH/flow
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Alternative name 2
CD8b, Lyt-3, T-Cell, Cytotoxic/Suppressor, Clone: YTS 156.7, Rat Mab antibody-Mouse, FITC; frozen, IH/flow
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Alternative name 3
CD8b, Lyt-3, T-Cell, Cytotoxic/Suppressor, Clone: YTS 156.7, Rattus norvegicus Mab antibody-Mus musculus, FITC; frozen, IH/flow
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Alternative name 4
anti-CD8b Lyt-3 T-Cell Cytotoxic/Suppressor Clone: YTS 156.7
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Alternative name 5
CD8b Lyt-3 T-Cell Cytotoxic/Suppressor Clone: YTS 156.7
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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Additional description
For cells, cell lines and tissues in culture till half confluency. Kinase, breast cancer tumor, HRAS like - suppressor protein make genetic suppression restoring the phenotype seen prior to the original background in biological pathways.
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Conjugation
Anti-FITC Antibody
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
YARS1
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Short name
CD8b, Lyt-3, T- , Cytotoxic/Suppressor, Clone: YTS 156 7, Mouse Monoclonal antibody-Mouse, FITC; frozen, IH/
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Technique
Mouse monoclonal, Antibody, frozen, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Label
FITC
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Species
Mouse, Mouses, Rats
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Alternative name
CD8b molecule, Lyt-3, T-cellular, Cytotoxic/Suppressor, clonality: YTS 156.7, Rat Mouse monoclonal (antibody to-)-Mouse, fluorecein; -25°C frozen, IH/flow
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD8b molecule, CD8B and IDBG-60041 and ENSG00000172116 and 100996919,926, MHC class I protein binding, Extracellular, Cd8b1 and IDBG-155547 and ENSMUSG00000053044 and 12526, CD8B and IDBG-634906 and ENSBTAG00000008956 and 508633
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Tissue
cell, frozen
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