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Synonyms
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, A2M, CPAMD5, FWP007
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Alternative_names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, A2M, CPAMD5, FWP007
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Description
A broad-spectrum protease-binding protein
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Recombinant
No
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Source
Human Plasma
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Molecular Weight
725 kDa (Homotetramer, Subunit size: 180 kDa)
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 100 mM Na Phosphate, pH 7.2.
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Reconstitution Instructions
In water or aqueous buffer
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Background Information
Alpha 2 Macroglobulin (A2M) is a plasma protease inhibitor which has been shown to exist in two forms. The Slow Form of A2M (S-A2M) is the form which possesses the ability to bind and inhibit proteases by a “trap” method. The Fast Form of A2M (F-A2M) is generated when S-A2M undergoes a conformational change due to either entrapment of a protease in the A2M bait region, or chemical cleavage of an internal thiol ester bond located near the bait region. F-A2M does not possess the ability to bind and inhibit protease activity. F-A2M is rapidly taken up by the liver, with a half-life of 2-4 minutes. In vivo, F-A2M typically represents only 0.17–0.7% of the total A2M in blood plasma of adults. The F-A2M plasma concentration is, however, increased in many disease states including pancreatitis, multiple sclerosis and sepsis. F-A2M has also been implicated in the inhibition of amyloid formation associated with Alzheimer’s disease and spongiform encephalopathy.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional description
The Alpha 2 Macroglobulin, Plasma, Fast Form is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
