Target of this antibody:
PLC gamma 2
Alternative names of antibody target:
PLC2 , PLC148 , PLC-II , PLC-148, inositoltrisphosphohydrolase
PLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLCβ, PLCg, PLCd, and PLCe. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCg2 is engaged in antigen-dependent signaling in B-cells and collagen-dependent signaling in platelets.
This antibody reacts with:
Synthetic peptide surrounding amino acid 1252 of human PLCg2
Formulation of this antibody:
100 µg (0.5 mg/ml) protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 139 kDa human PLCg2. Jurkat cell lysate can be used as a positive control. Reactivity to other species has not been determined.
Long gene name phospholipase C beta 1
Synonyms gene name
- phospholipase C, beta 1 (phosphoinositide-specific)
- phosphoinositide phospholipase C
Discovery year 2001-06-21
Entrez gene record 23236
- C2 domain containing phospholipases
Havana BLAST/BLAT OTTHUMG00000031849