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Also known as
Actin, alpha, Smooth Muscle
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Other names
actin, alpha 2, smooth muscle, aorta, isoform CRA_a; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS
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Gene name synonims
ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS
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Other gene names
ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a,k
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Clone
0.N.5
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Bovine, Chicken, Human (Homo sapiens), Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein A affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Glycerol free. Also available with BSA and azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
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Description
The Actin, alpha, Smooth Muscle (BSA, Glycerol & ) Antibody is a α- or alpha protein sometimes glycoprotein present in blood. Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request. Recently MBS Monoclonals made this Actin, alpha, Smooth Muscle (BSA, Glycerol & ) Antibody available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
