Anti-NIFK (Thr230) (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-10401R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  NK Thr230
• Specificity
  This antibody reacts specifically with NIFK (Thr230)
• Modification
  Phosphorylation
• Modification site
  Thr230
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human NIFK/MKI67IP around the phosphorylation site of Thr230
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009].
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  NK phospho T230; NK phospho Thr230; p-NKT230; p-NKThr230; hNK; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein; MKI67IP; NOPP34; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI 67; pKi67; MK67I_HUMAN.
• Also known as
  NIFK (Thr230) Polyclonal Antibody
• Other name
  Anti-NIFK (Thr230) Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  NIFK   Thr230  
• Gene symbol
  NIFK-AS1, NIFK
• Short name
  Anti-NIFK (Thr230)
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  NIFK (Thr230) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:27385
• Gene
  NIFK-AS1
• Long gene name
  NIFK antisense RNA 1
• Locus
  2q14.3
• Discovery year
  2013-10-10
• Entrez gene record
  254128
• Pubmed identfication
  • 12477932
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000153332

Gene info

• Identity
  HGNC:17838
• Gene
  NIFK
• Long gene name
  nucleolar protein interacting with the FHA domain of MKI67
• Synonyms gene
  • MKI67IP
• Synonyms gene name
  • MKI67 (FHA domain) interacting nucleolar phosphoprotein
• Synonyms
  • Nopp34
  • hNIFK
• Synonyms name
  • nucleolar protein interacting with the FHA domain of pKi-67
• GenBank acession
  • AB044971
• Locus
  2q14.3
• Discovery year
  2002-05-03
• Entrez gene record
  84365
• Pubmed identfication
  • 11342549
  • 16244663
  • 25826659
• RefSeq identity
  • NM_032390
• Classification
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000131442

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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