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Rabbit Anti Human STAT3 Polyclonal

Rabbit Anti Human STAT3 Polyclonal is available 8 times from Stressmark antibodies labs

SPC-1334D-ALP | Rabbit Anti-Human STAT3 Polyclonal: Alkaline Phosphatase size: 100 µl | 402.29 USD

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SPC-1334D-A390 | Rabbit Anti-Human STAT3 Polyclonal: ATTO 390 size: 100 µl | 411.49 USD

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SPC-1334D-A565 | Rabbit Anti-Human STAT3 Polyclonal: ATTO 565 size: 100 µl | 410.34 USD

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SPC-1334D-A633 | Rabbit Anti-Human STAT3 Polyclonal: ATTO 633 size: 100 µl | 410.34 USD

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SPC-1334D-A680 | Rabbit Anti-Human STAT3 Polyclonal: ATTO 680 size: 100 µl | 410.34 USD

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SPC-1334D-A700 | Rabbit Anti-Human STAT3 Polyclonal: ATTO 700 size: 100 µl | 410.34 USD

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SPC-1334D-BI | Rabbit Anti-Human STAT3 Polyclonal: Biotin size: 100 µl | 404.59 USD

Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test StressMark antibodies supplies antibodies that are for research of human proteins.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation Biotinylated
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetSTAT3
Short name Rabbit Anti- STAT3 Polyclonal:
Technique anti-Human, Polyclonal, Rabbit, anti
Label Biotin
Alternative name production species: rabbit antibody to-H. sapiens signal transducer and activator on transcription 3 (acute-phase response factor) polyclonal: biotinilated
Alternative to gene target signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541
1. Gene info
Identity11364
GeneSTAT3
Long gene name signal transducer and activator of transcription 3
Synonyms gene name
  • signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms
GenBank acession
Locus17q21.2
Discovery year 1995-11-08
Entrez gene record6774
Pubmed identfication
RefSeq identity
Classification
  • SH2 domain containing
Havana BLAST/BLATOTTHUMG00000150645
MeSH Data
Concept
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Additional information
Product images
    #Human#.jpg

SPC-1334D-RPE | Rabbit Anti-Human STAT3 Polyclonal: RPE size: 100 µl | 405.74 USD

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PubMed number Refer to PubMed
Net weight (g) 0.1
Recommended buffer for storage PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Antibody's reactivity Human, Mouse, Rat
Warning information Non-hazardous
Representative figure legend Western blot analysis of Human K562 cell lysates showing detection of ~86kDa STAT3 protein using Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334) at 1:1000. Predicted/Observed Size: ~86kDa. Rabbit Anti-STAT3 Antibody used in Western blot (WB) on K562 cell lysates (SPC-1334)
Raised in Rabbit
NCBI number NP_644805.1
Gene number 6774
Released date 6/Sep/2016
Immunogen A synthesized peptide derived from human STAT3
Antibody's other name Acute Phase Response Factor Antibody, ADMIO Antibody, APRF Antibody, DNA binding protein APRF Antibody, HIES Antibody, Signal transducer and activator of transcription 3 Antibody, STAT 3 Antibody
Protein number P40763
Antibody's recommended dilutions for use WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Tested applications To be tested
Primary research fields Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Total weight (kg) 1.4
Stock availabilit In Stock
Scientific context Stat3 is an important transciption factor for many cytokines and growth factor receptors. It is constitutively activated in a number of human tumors and has oncogenic potential and anti-apoptotic activites.
Antibody's target Human STAT3
Certificate of analysis A 1:1000 dilution of SPC-1334 was sufficient for detection of STAT3 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Tissue specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Antibody's full description Rabbit Anti-Human STAT3 Polyclonal
Country of production Canada
Antibody's purified from Peptide Affinity Purified
Antibody's concentration See included datasheet or contact our support service
Tested reactivity To be tested
Antibody in cell Cytoplasm, Nucleus
Antibody's category Polyclonal Antibodies
Bibliography 1. Darnell J.E., et al. (1994) Science. 264: 1415-1421. 2. Garcia R. and Jove R. (1998) J Biomed Sci. 5: 79-85. 3. Bromberg J.F., et al. (1999) Cell. 98: 295-303.
Verified applications WB, IHC
Antibody's specificity Detects ~85kDa, endogenous levels of total STAT3
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Host Rabbit
Species Human
Alternative technique polyclonals
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