Peroxiredoxin 5 Antibody (PRDX5)

• Catalog number
  R30936
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Peroxiredoxin 5 (PRDX5)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Peroxiredoxin 5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P30044
• Purity
  Antigen affinity
• Description
  Peroxiredoxin 5, also known as PRX5 and PRDX5, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that Peroxiredoxin 5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. Peroxiredoxin 5 plays a protective role against oxidative stress in human cartilage.
• Immunogen
  An amino acid sequence from the C-terminus of human PRDX5 (DDSLVSIFGNRRLKRF) was used as the immunogen for this Peroxiredoxin 5 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the Peroxiredoxin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Peroxiredoxin   PRDX5  
• Gene symbol
  PRDX5
• Short name
  Anti-Peroxiredoxin 5 (PRDX5)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Peroxiredoxin 5 (PRDX5)
• Alternative technique
  antibodies
• Alternative to gene target
  peroxiredoxin 5, ACR1 and AOEB166 and B166 and HEL-S-55 and PLP and PMP20 and PRDX6 and prx-V and PRXV, PRDX5 and IDBG-53649 and ENSG00000126432 and 25824, peroxynitrite reductase activity, nuclei, Prdx5 and IDBG-136962 and ENSMUSG00000024953 and 54683, PRDX5 and IDBG-643929 and ENSBTAG00000008648 and 282885

Gene info

• Identity
  HGNC:9355
• Gene
  PRDX5
• Long gene name
  peroxiredoxin 5
• Synonyms
  • ACR1
  • AOEB166
  • MGC142285
  • PRXV
  • PMP20
  • B166
  • PRDX6
  • PLP
  • SBBI10
  • MGC117264
  • MGC142283
• Synonyms name
  • antioxidant enzyme B166
  • thioredoxin peroxidase PMP20
  • peroxisomal antioxidant enzyme
  • TPx type VI
  • liver tissue 2D-page spot 71B
  • Alu co-repressor 1
• GenBank acession
  • AF197952
• Locus
  11q13.1
• Discovery year
  2000-07-31
• Entrez gene record
  25824
• Pubmed identfication
  • 10514471
  • 10521424
• RefSeq identity
  • NM_181651
• Classification
  • Peroxiredoxins
• VEGA ID
  OTTHUMG00000168805

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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