V-ATPase, B, vacuolar H+-ATPase subunit B
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Catalog numberAS14 2775
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PricePlease ask
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Size50 µg
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Available ordering formatInquire
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ImmunogenKLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum in PBS pH 7.4
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB)
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Connected productscollection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa)AS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 1000 with ECL (WB)
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Molecular weight expected аpparent53 | 57 kDa (Vigna radiata)
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Verified reactivityArabidopsis halleri, Arabidopsis thaliana, Nicotiana tabaccum , Thellungiella salsuginea, Vigna radiata
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Possible reactivityAcetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Glycine soja, Gossypium mexicanum, Halostachys caspica, Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrella patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
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Referencesto be added when available, antibody released in June 2015
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Scientific contextV-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolar H+-ATPase subunit B, V-ATPase 57 kDa subunit.
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NotesAntibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of vacuolar membranes can be found here.
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Protein numberQ683E8 , Q9SZN1 , Q8W4E2
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TAIR numberAT1G76030,AT4G38510,AT1G20260
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Gene target
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Gene symbolATP6V0B, ATP6V1B2
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Short nameV-ATPase, B, vacuolar H+-ATPase subunit B
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Alternative nameV-ATPase, B, vacuolar H+-ATPase functionnal sequence B
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Gene info
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Identity
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Gene
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Long gene nameATPase H+ transporting V0 subunit b
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Synonyms gene
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Synonyms gene name
- ATPase, H+ transporting, lysosomal (vacuolar proton pump) 21kD
- ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c''
- ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-type ATPase subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameATPase H+ transporting V1 subunit B2
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Synonyms gene
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Synonyms gene name
- ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2
- ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
- ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-type ATPase subunits
- ATPase F1/V1 alpha/A and beta/B subunit family
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VEGA ID