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Cytidine deaminase, human recombinant

Cytidine deaminase, human recombinant is available 1 time from Biovision labs

7363-100 | Cytidine deaminase, human recombinant size: 100 ug | 398.32 USD

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Synonyms CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
Alternative_names CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
Description An enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥90%
Assay SDS-PAGE
Molecular Weight 18.3 kDa (166 aa, 1-146 aa + His Tag).
Storage Temp. -80°C
Shipping Dry Ice
Shelf Life 12 months
Concentration 0.5 mg/ml
Appearance Liquid
Physical form description 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.
Background Information CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetCytidine deaminase
Short name Cytidine deaminase, recombinant
Technique Recombinant
Species Human
Alternative name Cytidine deaminase, H. sapiens recombinant
Alternative technique rec
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