Mouse Monoclonal [clone 10G12] (IgG2a,k) to Human LIG1 / DNA Ligase 1

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Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

Monoclonal 10G12] IgG2a LIG1 DNA Ligase
Gene symbol

LIG1, LRIG1, PRKDC, HLA-DOA
Short name

Mouse Monoclonal [clone 10G12] (IgG2a k) LIG1 / DNA Ligase 1
Technique

Mouse monoclonal, Mouse, dna, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
Host

mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
Isotype

IgG2a
Species

Human, Humans, Mouses
Alternative name

Mouse monoclonal [clonality 10G12] (IgG2a,k) to H. sapiens LIG1 / Desoxyribonucleic acid Ligase 1
Alternative technique

murine
Clone name

10G12

Identity

HGNC:6598
Gene

LIG1
Long gene name

DNA ligase 1
Synonyms gene name
- ligase I, DNA, ATP-dependent
Synonyms name
- DNA ligase I
Locus

19q13.33
Discovery year

1991-05-09
Entrez gene record

3978
Pubmed identfication
- 1733856
RefSeq identity
- NM_000234
Classification
- Nucleotide excision repair
- DNA ligases
VEGA ID

OTTHUMG00000183187
Locus Specific Databases
- LIG1base: Mutation registry for DNA ligase I deficiency
- LRG_78

Identity

HGNC:17360
Gene

LRIG1
Long gene name

leucine rich repeats and immunoglobulin like domains 1
Synonyms
- LIG-1
- DKFZP586O1624
- LIG1
Synonyms name
- ortholog of mouse integral membrane glycoprotein LIG-1
- leucine-rich repeat protein LRIG1
GenBank acession
- AB050468
Locus

3p14.1
Discovery year

2002-08-23
Entrez gene record

26018
Pubmed identfication
- 11414704
- 12234026
RefSeq identity
- NM_015541
Classification
- I-set domain containing
VEGA ID

OTTHUMG00000158727

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data