Blasticidin solution, 10mg/ml in 20mM HEPES
-
Catalog number
B006-20 ml
-
Price
Please ask
-
Size
20ml
-
-
Chemical formula
C17H26N8O5*HCl
-
CAS number
3513-03-9
-
Transportation conditions
dry ice
-
Storage Temp
Upon receipt place and keep the vial frozen at -20 degrees Celcius.
-
Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
-
Description
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
-
Gene target
-
Short name
Blasticidin , 10mg/ in 20mM
-
Alternative name
Blasticidin solution, 10mg/milliliter in 20mM HEPES
-
Similar products