Mouse Anti-Human SNAP-25 Monoclonal IgG1, Clone SP-12

  • Catalog number
    SMC-180D-A680
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    SP-12
  • Immunogen
    Raised against a crude synaptic preparation from the post mortem human brain
  • Antibody s full description
    Mouse Anti-Human SNAP25 Monoclonal IgG1 Antibody, Clone: SP-12: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Bdr Antibody, RIC4 Antibody, SNAP25 Antibody, SNAP-25B Antibody, SNP25 Antibody, SUP Antibody, super protein Antibody, synaptosomal associated 25kDa protein Antibody
  • Raised in
    Mouse
  • Antibody s target
    SNAP25
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers, Cell Signaling, Trafficking, Cancer
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, ELISA, AM
  • Antibody s reactivity
    Human, Mouse, Rat, Cat, Pig
  • Antibody s dilutions
    WB (1:1000), IHC (1:100), ICC/IF (1:1000) ELISA (1:5000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~29kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Synaptosomal-associated protein 25 (SNAP-25) is a membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3).
  • Bibliography
    1. Hodel A. (1998) Int J. Biochem Cell Biol. 30(10): 1069-1073. 2. Sudhof T.C., and Rizo J. (2002) Nat Rev Neurosci. 3(8): 641-653. 3. Puffer E.B., et al. (2001) Biochemistry. 40(31): 9374-9378.
  • Released date
    30-Jun-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_570824.1
  • Gene number
    6616
  • Protein number
    P60880
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (SMC-180). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (SMC-180) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive muscle, epidermal and dermal staining. Western Blot analysis of Human Cell lysates showing detection of SNAP25 protein using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (SMC-180). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (SMC-180) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SNAP25 Antibody [SP-12] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-180) Mouse Anti-SNAP25 Antibody [SP-12] used in Western Blot (WB) on Human Cell lysates (SMC-180)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    SNAP25, SNAP25-AS1, SFTPA1, SPATA16, SNORD116-25, ZNF211, SNORD114-25, SNORD115-25, ERVW-25, IGKV3D-25
  • Short name
    Mouse Anti- SNAP-25 Monoclonal IgG1, Clone SP-12
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 680
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman SNAP-25 monoclonal IgG1, clonality SP-12
  • Alternative technique
    murine, antibodies
  • Clone name
    SP-12
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    endogenous retrovirus group W member 25
  • Synonyms gene name
    • endogenous retrovirus group W, member 25
  • GenBank acession
  • Locus
  • Discovery year
    2013-10-21
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 3D-25 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 3D-25
    • immunoglobulin kappa variable 3D-25 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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