Mouse Anti-Human SNAP-25 Monoclonal IgG1, Clone SP-12
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Catalog numberSMC-180D-A680
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PricePlease ask
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Size100 µg
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CloneSP-12
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ImmunogenRaised against a crude synaptic preparation from the post mortem human brain
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Antibody s full descriptionMouse Anti-Human SNAP25 Monoclonal IgG1 Antibody, Clone: SP-12: ATTO 680
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameBdr Antibody, RIC4 Antibody, SNAP25 Antibody, SNAP-25B Antibody, SNP25 Antibody, SUP Antibody, super protein Antibody, synaptosomal associated 25kDa protein Antibody
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Raised inMouse
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Antibody s targetSNAP25
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers, Cell Signaling, Trafficking, Cancer
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, ELISA, AM
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Antibody s reactivityHuman, Mouse, Rat, Cat, Pig
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Antibody s dilutionsWB (1:1000), IHC (1:100), ICC/IF (1:1000) ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~29kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSynaptosomal-associated protein 25 (SNAP-25) is a membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3).
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Bibliography1. Hodel A. (1998) Int J. Biochem Cell Biol. 30(10): 1069-1073. 2. Sudhof T.C., and Rizo J. (2002) Nat Rev Neurosci. 3(8): 641-653. 3. Puffer E.B., et al. (2001) Biochemistry. 40(31): 9374-9378.
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Released date30-Jun-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_570824.1
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Gene number6616
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Protein numberP60880
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (SMC-180). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (SMC-180) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive muscle, epidermal and dermal staining. Western Blot analysis of Human Cell lysates showing detection of SNAP25 protein using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (SMC-180). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (SMC-180) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SNAP25 Antibody [SP-12] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-180) Mouse Anti-SNAP25 Antibody [SP-12] used in Western Blot (WB) on Human Cell lysates (SMC-180)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNAP25, SNAP25-AS1, SFTPA1, SPATA16, SNORD116-25, ZNF211, SNORD114-25, SNORD115-25, ERVW-25, IGKV3D-25
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Short nameMouse Anti- SNAP-25 Monoclonal IgG1, Clone SP-12
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 680
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman SNAP-25 monoclonal IgG1, clonality SP-12
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Alternative techniquemurine, antibodies
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Clone nameSP-12
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Gene info
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Identity
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Gene
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Long gene namesynaptosome associated protein 25
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Synonyms gene
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Synonyms gene name
- synaptosomal-associated protein, 25kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year1995-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SNAREs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSNAP25 antisense RNA 1
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Synonyms gene name
- SNAP25 antisense RNA 1 (non-protein coding)
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GenBank acession
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Locus
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Discovery year2012-08-09
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesurfactant protein A1
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Synonyms gene
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Synonyms gene name
- surfactant, pulmonary-associated protein A1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Collectins
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namespermatogenesis associated 16
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-01-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-25
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 211
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-29
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-25
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-25
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 25
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Synonyms gene name
- endogenous retrovirus group W, member 25
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-25 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-25
- immunoglobulin kappa variable 3D-25 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data