Recombinant Mouse ITK Kinase Protein (aa 351-619, His & GST tag)

• Catalog number
  PKSM040718-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  Emt;Tcsk;Tsk
• Activity
  NA
• Sequence
  Arg 351-Leu 619
• Fusion tag
  N-His & GST
• Accession
  Q03526
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 80 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  58.4 kDa
• AP Mol Mass
  58 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 0.5mM GSH
• Background
  IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain and 1 SH3 domain. As an intracellular kinase which expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is hought to play a role in T-cell proliferation and differentiation. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in regulation of the adaptive immune response. efects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ITK   Kinase   Protein   351-619   His   GST   tag  
• Gene symbol
  MGST3, ITK, MIR619
• Short name
  Recombinant Mouse ITK Kinase Protein (aa 351-619, His & GST tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse IL2-inducible T-cell kinase phosphorylation catalyst Protein (aa 351-619, histidine & GST detection labelled)
• Alternative technique
  rec, murine
• Alternative to gene target
  IL2-inducible T-cell kinase, EMT and LPFS1 and LYK and PSCTK2, ITK and IDBG-55348 and ENSG00000113263 and 3702, transferase activity, Cell surfaces, Itk and IDBG-165583 and ENSMUSG00000020395 and 16428, ITK and IDBG-646384 and ENSBTAG00000000306 and 522311

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:6171
• Gene
  ITK
• Long gene name
  IL2 inducible T cell kinase
• Synonyms gene name
  • IL2-inducible T-cell kinase
  • IL2 inducible T-cell kinase
• Synonyms
  • EMT
  • PSCTK2
  • LYK
• GenBank acession
  • D13720
• Locus
  5q33.3
• Discovery year
  1995-12-18
• Entrez gene record
  3702
• Pubmed identfication
  • 8364206
• Classification
  • Tec family tyrosine kinases
  • SH2 domain containing
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000130245
• Locus Specific Databases
  • LRG_189

Gene info

• Identity
  HGNC:32875
• Gene
  MIR619
• Long gene name
  microRNA 619
• Synonyms gene
  • MIRN619
• Synonyms
  • hsa-mir-619
• Locus
  12q24.11
• Discovery year
  2006-05-17
• Entrez gene record
  693204
• RefSeq identity
  • NR_030350
• Classification
  • MicroRNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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