igScript™ Probe-Based RT-qPCR Kit, 50 rnx
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Catalog number
4243
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Price
Please ask
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Size
50 reactions
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Description
IgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions. IgScript™ first strand cDNA synthesis kit includes 5x IgScript™ master mix which contains IgScript™ Reverse Transcriptase, RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nucleasefree water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-50 ºC. Probe-based qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA. This 2x master mix includes proprietary buffer, Taq DNA polymerase, low ROX reference dye, MgCl2, dNTPs, stabilizers and enhancers.
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Shipping Condition
Ships with Dry Ice
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Additional description
any of various testing devices or substances as (1) a pointed metal tip for making electrical contact with a circuit element being checked (2) a usually small object that is inserted into something so as to test conditions at a given point (3) a device used to penetrate or send back information especially from outer space or a celestial body (4) a device (as an ultrasound generator) or a substance (as radioactively labeled DNA) used to obtain specific information for diagnostic or experimental purposes
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Gene target
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Gene symbol
SNRPN
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Short name
igScript™ Probe-Based RT-qPCR Kit, 50 rnx
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Technique
probe, probes
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Alternative name
igScript™ Probe-Based Reverse transcription quantitative PCR test kit reagent, 50 rnx
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Alternative technique
probes, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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