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Category
Primary Antibodies
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Long description
DDH is a member of aldo-keto reductase superfamily1,2, which catalyzes reduction of aldehyde or ketone to a corresponding alcohol by using NADH or NADPH as a cofactor. In liver, the enzyme is abundantly located in the cytoplasm as a monomeric 34-36 kDa protein3,4. Interestingly, by differential display Shen et al.5 has shown that overexpression of DDH could be identified in ethacrynic acid-induced drug-resistant human colon cancer cells. Detection of DDH overexpression in drug-resistant human stomach cancer cells, which were selected by the gradual adaptation to daunorubicin, further suggested that DDH might be associated with the drug-resistance in cancer cells6._x000B_In a recent study, DDH expression was further shown to have prognostic significance in patients with NSCLC. By using an immunohistochemical method to determine DDH expression in surgical specimens, DDH expression was identified in patients with leukemia, lung cancer, esophageal cancer, transitional cancer and breast cancer. Furthermore, overexpression of DDH was confirmed by immunoblotting and in situ hybridization. Correlation between clinicopathological parameters and DDH expression as well as the prognostic significance of DDH expression in patients with advanced cancer was indicated.
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Antibody come from
n/a
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Other description
Provided as whole ascites
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Clone
T101
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Antigen antibody binding interaction
Mouse anti Dihydrodiol Dehydrogenase Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Porcine (most mammalian species)
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry, Immunofluorescense, Enzyme Immunoassay_x000B_
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Antibody s suited for
¥ 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips._x000B_¥ Automated plate washer, wash bottle or multichannel dispenser for washing._x000B_¥ 2 liter graduated cylinder._x000B_¥ Deionized or distilled H2O._x000B_¥ 0.2 µm syringe filter and syringe._x000B_¥ Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450/595 nm or 450/540 nm. A single wavelength of 450 nm can also be used.
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Storage
-20ºC
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Relevant references
1. p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Flatt, P.M., et al., 'Cancer Lett.' 2000, 156, 63-73._x000B__x000B_2. The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. Venot, C., et al., 'EMBO J.' 1998, 17, 4668-4679._x000B__x000B_3. Regulation of p53 downstream genes. el-Deiry, W.S., Semin. 'Cancer Biol.' 1998, 8, 345-367._x000B__x000B_4. Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1 Gad Asher, Joseph Lotem, Batya Cohen, Leo Sachs, and Yosef Shaul. 1188-1193, _x000B_PNAS,' January 30, 2001, vol.98, no.3._x000B__x000B_5. Characterization of distinct consecutive phases in non-genotoxic p53-induced apoptosis of Ewing tumor cells and the rate-limiting role of caspase 8. Kovar H, Jug G, Printz D, Bartl S, Schmid G, Wesirska-Gadek J. 'Oncogene' 2000 Aug 19:4096-107._x000B__x000B_6. Tumor-derived mutations within the DNA-binding domain of p53 that phenotypically resemble the deletion of the proline-rich domain. Roth J, Koch P, Contente A, Dobbelstein M. 'Oncogene' 2000 Mar 19:1834-42._x000B__x000B_7. The requirement for the p53 priline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. Corinne venot, Michel Maratrat, Christine Dureil, emmanuel Conseiller, Laurent Bracco and Laurent Debussche. 'EMBO Journal' Vol.17 No.16 pp.4668-4679, 1998.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
