CD28 monoclonal antibody
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Catalog number12-597-C100
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PricePlease ask
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Size100ug
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Long nameHamster Monoclonal Antibody against Mouse CD28, Clone: 37.51
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AntigenCD28 (mouse)
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Species reactivityMouse (Mus musculus)
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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Clone37.51
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ApplicationsFC, IP, IHC(F), ICC, FUNC
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ImmunogenMouse T cell lymphoma EL-4 cells_x000D_
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Format conjugationlow endotoxin, unconjugated
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SpecificityThe Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells._x000D_
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Research areaCD and Related Antigens & MHC (Rodent)
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Concentration1 mg/ml
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Storage bufferAzide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
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Shipping and storageHamster Monoclonal Antibody against Mouse CD28, Clone: 37.51 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells._x000D_
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by protein-G affinity chromatography
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ReferencesContact us for the most recent list of references
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolCD28
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Short nameAnti-CD28 Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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IsotypeIgG
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Alternative nameHamster Monoclonal to CD28 (mouse)
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Alternative techniqueantibodies
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Alternative to gene targetCD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
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Gene info
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Identity
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Gene
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Long gene nameCD28 molecule
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Synonyms gene name
- CD28 antigen (Tp44)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data