Chemerin, human recombinant
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Catalog number
4002-1000
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Price
Please ask
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Size
1 mg
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Synonyms
RARRES2; HP10433; TIG2
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Alternative_names
RARRES2; HP10433; TIG2, G-protein coupled receptor ChemR23, G-protein coupled receptor DEZ
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Description
A chemoattractant and a ligand for G-protein coupled receptors
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Biological activity
Recombinant human Chemerin has full biological activity when compared standards. The ED₅₀, as determined by its ability to chemoattract human Chem23R transfected BaF3 mouse pro-B cells, is typically 4-20 ng/mL.
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Results
4-20 ng/mL
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Molecular Weight
16.0 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized from 0.2% TFA.
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Reconstitution Instructions
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
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Background Information
Chemerin is a chemoattractant expressed in white adipose, liver and lung tissues. Chemerin is a ligand for the G-protein coupled receptor known as ChemR23 (or chemokine-like receptor-1), which is expressed mainly on dendritic cells, macrophages and some adipocytes. Recombinant human Chemerin, produced in E.coli, is a non-glycosylated protein containing 138 amino acids and having a total molecular mass of 16 kDa.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CMKLR1, CMKLR2, RARRES2
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Short name
Chemerin, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Chemerin, H. sapiens Rec.
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Alternative technique
rec
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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