Mouse Anti-Rat GABA-B Receptor 1 Monoclonal IgG1, Clone S93A-49

• Catalog number
  SMC-403D-A390
• Price
  Please ask
• Size
  100 µg

• Clone
  S93A-49
• Immunogen
  Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
• Antibody s full description
  Mouse Anti-Rat GABA B Receptor 1 Monoclonal IgG1 Antibody, Clone: S93A-49: ATTO 390
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  GABA-B receptor 1 Antibody, GABA-B-R1 Antibody, GABR1_Human Antibody, Gamma aminobutyric acid receptor 1 Antibody, GB1 Antibody, GPRC3A Antibody
• Raised in
  Mouse
• Antibody s target
  GABA B Receptor 1
• Primary research fields
  Neuroscience, Neurotransmitter Receptors, GABA Receptors, GABAB Receptors
• Brandname
  None
• Antibodies applications
  WB, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~115kDa. No cross-reactivity against GABA(B)R2.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-403 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
• Bibliography
  1. Jones K.A,. et al. (2000) Neuropsychopharmacology 23: S41-9. 2. Duthey B., et al. (2002) J Biol Chem. 277: 3236-41. 3. Kaupmann K., et al. (1997) Nature 386: 239-46.
• Released date
  16-May-2012
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_112290.2
• Gene number
  81657
• Protein number
  Q9Z0U4
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone S93A-49 (SMC-403). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (SMC-403) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-B Receptor 1 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49 (SMC-403). Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody (SMC-403) at 1:1000. Mouse Anti-GABA-B Receptor 1 Antibody [S93A-49] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-403) Mouse Anti-GABA B Receptor 1 Antibody [S93A-49] used in Western Blot (WB) on Rat brain membrane lysate (SMC-403)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  GABA-B   Receptor   Monoclonal   IgG1   S93A-49  
• Gene symbol
  IGLV9-49, IGHV3-49, IGHVII-49-1, NDUFS2, ABAT, IFIT3, SCCPDH, RNU6-49P, ZNF142, SNRPB
• Short name
  Mouse Anti- GABA-B Receptor 1 Monoclonal IgG1, Clone S93A-49
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 390
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat GABA-B Receptor 1 monoclonal IgG1, clonality S93A-49
• Alternative technique
  murine, antibodies
• Clone name
  S93A-49

Gene info

• Identity
  HGNC:5933
• Gene
  IGLV9-49
• Long gene name
  immunoglobulin lambda variable 9-49
• GenBank acession
  • Z73675
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28773
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151045

Gene info

• Identity
  HGNC:5607
• Gene
  IGHV3-49
• Long gene name
  immunoglobulin heavy variable 3-49
• GenBank acession
  • M99676
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28423
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151967

Gene info

• Identity
  HGNC:5683
• Gene
  IGHVII-49-1
• Long gene name
  immunoglobulin heavy variable (II)-49-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-49-1
  • immunoglobulin heavy variable (II)-49-1 pseudogene
• Synonyms
  • IGHV(II)-49-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28364
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151974

Gene info

• Identity
  HGNC:7708
• Gene
  NDUFS2
• Long gene name
  NADH:ubiquinone oxidoreductase core subunit S2
• Synonyms gene name
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Q reductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
• Synonyms
  • CI-49
• Synonyms name
  • complex I 49kDa subunit
  • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
• GenBank acession
  • BC008868
• Locus
  1q23.3
• Discovery year
  1992-06-30
• Entrez gene record
  4720
• Pubmed identfication
  • 1832859
  • 9585441
• RefSeq identity
  • NM_004550
• Classification
  • NADH:ubiquinone oxidoreductase core subunits
• VEGA ID
  OTTHUMG00000034344

Gene info

• Identity
  HGNC:23
• Gene
  ABAT
• Long gene name
  4-aminobutyrate aminotransferase
• Synonyms
  • GABAT
  • GABA-T
• Synonyms name
  • 4-aminobutyrate transaminase
  • gamma-aminobutyrate aminotransferase
  • GABA transaminase
• GenBank acession
  • L32961
• Locus
  16p13.2
• Discovery year
  1996-03-13
• Entrez gene record
  18
• Pubmed identfication
  • 7721088
• RefSeq identity
  • NM_020686
• VEGA ID
  OTTHUMG00000048201

Gene info

• Identity
  HGNC:5411
• Gene
  IFIT3
• Long gene name
  interferon induced protein with tetratricopeptide repeats 3
• Synonyms gene
  • IFIT4
• Synonyms gene name
  • interferon-induced protein with tetratricopeptide repeats 4
  • interferon-induced protein with tetratricopeptide repeats 3
• Synonyms
  • ISG60
  • RIG-G
  • CIG-49
  • IFI60
  • GARG-49
  • IRG2
• GenBank acession
  • U52513
• Locus
  10q23.31
• Discovery year
  1998-08-13
• Entrez gene record
  3437
• Pubmed identfication
  • 9828129
  • 9391139
• RefSeq identity
  • NM_001549
• Classification
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000018708

Gene info

• Identity
  HGNC:24275
• Gene
  SCCPDH
• Long gene name
  saccharopine dehydrogenase (putative)
• Synonyms
  • CGI-49
  • NET11
• Locus
  1q44
• Discovery year
  2005-06-22
• Entrez gene record
  51097
• Pubmed identfication
  • 10810093
• RefSeq identity
  • NM_016002
• VEGA ID
  OTTHUMG00000040221

Gene info

• Identity
  HGNC:34293
• Gene
  RNU6-49P
• Long gene name
  RNA, U6 small nuclear 49, pseudogene
• Synonyms gene
  • RNU6-49
• Synonyms gene name
  • RNA, U6 small nuclear 49
• Locus
  Xp11.4
• Discovery year
  2008-06-10
• Entrez gene record
  106478923

Gene info

• Identity
  HGNC:12927
• Gene
  ZNF142
• Long gene name
  zinc finger protein 142
• Synonyms gene name
  • zinc finger protein 142 (clone pHZ-49)
• Synonyms
  • KIAA0236
  • pHZ-49
• GenBank acession
  • U09849
• Locus
  2q35
• Discovery year
  1993-02-11
• Entrez gene record
  7701
• RefSeq identity
  • NM_005081
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000154736

Gene info

• Identity
  HGNC:11153
• Gene
  SNRPB
• Long gene name
  small nuclear ribonucleoprotein polypeptides B and B1
• Synonyms gene
  • SNRPB1
• Synonyms
  • COD
  • SmB/SmB'
  • Sm-B/B'
  • snRNP-B
• Locus
  20p13
• Discovery year
  1988-11-28
• Entrez gene record
  6628
• Pubmed identfication
  • 1376292
• Classification
  • Small nucleolar RNA protein coding host genes
  • Sm spliceosomal proteins
• VEGA ID
  OTTHUMG00000031694

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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