SMAD Antibody (SMAD1-5)
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Catalog numberR32238
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenSMAD (SMAD1-5)
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.1-0.5ug/ml
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NotesOptimal dilution of the SMAD antibody should be determined by the researcher.
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Intented useThis SMAD antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ15797
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PurityAntigen affinity
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DescriptionSMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. It was concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-beta superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.
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ImmunogenAmino acids QPMDTNMMAPPLPSEINRGDVQAVAYEEPKH of human SMAD1-5 were used as the immunogen for the SMAD antibody.
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StorageAfter reconstitution, the SMAD antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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LocalizationNuclear and cytoplasmic
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMAD1-AS2, SMAD1-AS1, SMAD1, GARS1, MIR1302-5, PIRC23, PIRC22, PIRC20, PIRC21, HTR6
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Short nameAnti-SMAD (SMAD1-5)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to SMAD (SMAD1-5)
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Alternative techniqueantibodies
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Alternative to gene targetSMAD family member 1, BSP-1 and BSP1 and JV4-1 and JV41 and MADH1 and MADR1, SMAD1 and IDBG-39936 and ENSG00000170365 and 4086, transforming growth factor beta receptor, nuclei, Smad1 and IDBG-170516 and ENSMUSG00000031681 and 17125, SMAD1 and IDBG-629431 and ENSBTAG00000002835 and 540488
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Gene info
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Identity
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Gene
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Long gene nameSMAD1 antisense RNA 2
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GenBank acession
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Locus
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Discovery year2013-10-22
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Entrez gene record
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSMAD1 antisense RNA 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2013-10-22
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Pubmed identfication
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSMAD family member 1
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Synonyms gene
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Synonyms gene name
- MAD, mothers against decapentaplegic homolog 1 (Drosophila)
- SMAD, mothers against DPP homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SMAD family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglycyl-tRNA synthetase 1
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Synonyms gene
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Synonyms gene name
- Charcot-Marie-Tooth neuropathy 2D
- glycyl-tRNA synthetase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-03-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aminoacyl tRNA synthetases, Class II
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 23
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 22
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 20
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 21
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 6
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 6
- 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data