Smad3 (Ser423 + Ser425) Antibody, Cy5 Conjugated
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Catalog number
bs-3425R-Cy5
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Price
Please ask
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Size
0.1ml
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Long name
Smad3 (Ser423 + Ser425) Antibody, Cy5 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy5
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Smad3 Ser423 + Ser425
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Specificity
This is a highly specific antibody against Smad3 Ser423 + Ser425. These phosphorylation sites are homologous across the species listed.
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Modification
Phosphorylation
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Modification site
Ser423 + Ser425
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
380-425/425
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]
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Gene ID number
4088
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Swiss Prot
P84022
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
625,650nm/670nm
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Synonyms
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
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Additional conjugation
Cy5.5
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French translation
anticorps
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Gene target
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Short name
Anti-Smad3 Ser423 + Ser425
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Cy5
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Alternative name
Anti-Smad3 Ser423 + Ser425 PAb Cy5
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Alternative technique
antibodies
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Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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