Leukemia DEK-CAN Fusion Gene Real Time RT-PCR Kit

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Description

Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Group

PCR, polymerase chain reaction, RT-PCR mixes
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

Leukemia DEK-CAN Gene Real Time Kit
Gene symbol

DEK, NUP214
Short name

Leukemia DEK-CAN Real Time RT-PCR Kit
Technique

RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Leukemia DEK-CAN Fusion Gene Real Time Reverse transcription PCR test kit reagent
Alternative technique

rtpcrkits, kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Virus

leukemia

Identity

HGNC:8064
Gene

NUP214
Long gene name

nucleoporin 214
Synonyms gene name
- nucleoporin 214kD (CAIN)
- nucleoporin 214kDa
Synonyms
- CAIN
- CAN
- D9S46E
- N214
Synonyms name
- nuclear pore complex protein Nup214
- CAN protein, putative oncogene
GenBank acession
- X64228
Locus

9q34.13
Discovery year

1999-05-05
Entrez gene record

8021
Pubmed identfication
- 8108440
- 2370860
RefSeq identity
- NM_005085
Classification
- Nucleoporins
VEGA ID

OTTHUMG00000020816
Locus Specific Databases
- LRG_1383

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data