Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
For quantitative measurement of human IL-8 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
More gene info
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
heparin, EDTA or citrate
ELISA's sensitivity< 1pg/ml
Gen protein identity
the ELISA does not cross react with any other proteins except from closely related species
Expression system for standard: E.coli; Immunogen sequence: S28-S99
ELISA standard curve
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.003 + 0.170 + 0.321 + 0.594 + 1.121 + 1.878 + 2.552 + 2.779
chemokine (C-X-C motif) ligand 8
ELISA kit description
GENTAUR's human IL-8 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-8 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, S28-S99) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-8 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-8 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation
How the elisa works
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human IL-8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each human IL-8 standard solution and each sample be measured in duplicate.
(Ala-IL-8)77;_x000D_(Ser-IL-8)72;_x000D_3 10C;_x000D__x000D__x000D_3-10C;_x000D_AMCF I;_x000D_AMCF-I;_x000D_AMCFI;_x000D_b ENAP;_x000D_B-ENAP;_x000D_Beta-thromboglobulin-like protein;_x000D_C X C motif chemokine 8;_x000D_C-X-C motif chemokine 8;_x000D_Chemokine (C X C motif) ligand 8;_x000D_CXCL8 ;_x000D_Emoctakin;_x000D_GCP 1;_x000D_GCP-1;_x000D_GCP/IL-8 protein I;_x000D_GCP/IL-8 protein II;_x000D_GCP/IL-8 protein III;_x000D_GCP/IL-8 protein IV;_x000D_GCP/IL-8 protein V;_x000D_GCP/IL-8 protein VI;_x000D_GCP1 ;_x000D_Granulocyte chemotactic protein 1;_x000D_IL 8;_x000D_IL-8;_x000D_IL-8(1-77);_x000D_IL-8(9-77);_x000D_IL8;_x000D_IL8/NAP1 form I;_x000D_IL8/NAP1 form II;_x000D_IL8/NAP1 form III;_x000D_IL8/NAP1 form IV;_x000D_IL8/NAP1 form V;_x000D_IL8/NAP1 form VI;_x000D_IL8_HUMAN;_x000D_Interleukin 8;_x000D_K60;_x000D_LECT;_x000D_LUCT;_x000D_Lymphocyte derived neutrophil activating factor;_x000D_Lymphocyte-derived neutrophil-activating factor;_x000D_LYNAP;_x000D_MDNCF;_x000D_MDNCF-b;_x000D_MDNCF-c;_x000D_MONAP;_x000D_Monocyte derived neutrophil activating peptide;_x000D_Monocyte derived neutrophil chemotactic factor;_x000D_Monocyte-derived neutrophil chemotactic factor;_x000D_Monocyte-derived neutrophil-activating peptide;_x000D_NAF;_x000D_NAP 1;_x000D_NAP-1;_x000D_NAP1;_x000D_Neutrophil activating peptide 1;_x000D_Neutrophil activating protein 1;_x000D_Neutrophil-activating factor;_x000D_Neutrophil-activating protein 1;_x000D_Protein 3 10C;_x000D_Protein 3-10C;_x000D_SCYB8;_x000D_Small inducible cytokine subfamily B member 8;_x000D_T cell chemotactic factor;_x000D_T-cell chemotactic factor;_x000D_TSG 1;_x000D_TSG-1;_x000D_TSG1
ELISA kit detection limits
15.6pg/ml to 1000pg/ml(cellculturesupernatant)7.8pg/ml to 500pg/ml(serum,plasma)
There is no detectable cross-reactivity with other relevant proteins.
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-8 antibodies;1 ST0000-10;Lyophilized recombinant human IL-8 standard;10ng/tubex2 AR1107;Biotinylated anti- human IL-8 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Target of this ELISA
Natural and recombinant human IL-8
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);115;357;616;176;392;649 ELISA standard deviations 6.67;17.5;41.3;13.38;21.17;45.43 CV(%);5.8;4.9;6.7;7.6;5.4;7
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
IL-8 PicoKine ELISA Kit
ELISA kit, ELISA
antigen assay ELISA kit 1 Kit (96 Wells)
H. sapiens Interleukin-8 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832