Cobalt Colorimetric Assay Kit
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Catalog number
K505-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (475 nm) • Species reactivity- Mammalian • Applications- The assay is linear in the range 3 to 50 nmol (0.18-3 µg) Cobalt, with a detection limit of ~ 3 nmol (or ~15 µM, 0.18 µg).
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Sample Type
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes only 20 minutes • Fast and convenient • The BioVision Cobalt Assay kit takes advantage of the reaction of Co²ᶧ with mercaptoethanol in basic media.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals [1]. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co²ᶧ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The BioVision Cobalt Assay kit takes advantage of the reaction of Co²ᶧ with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe²ᶧ, Cu²ᶧ, Ni²ᶧ, Zn²ᶧ, Mn²ᶧ, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co²ᶧ in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Cobalt Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Cobalt colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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