PicoProbeTM Phosphatidylinositol Assay Kit (Fluorometric)

• Catalog number
  K750-100
• Price
  Please ask
• Size
  100 assays

• Description
  The first kit in the market for the detection of Phosphatidylinositol. Sensitive. Specific
• Summary
  Phosphatidylinositol Assay Kit (Fluorometric): Assay Kit for measuring Phosphatidylinositol in biological samples such as tissue/cell lysate and biological fluids. Detection limit: 15 pmol per sample. 100 assays.
• Detection Method
  Fluorometric (Ex/Em = 535/587 nm)
• Species Reactivity
  All
• Applications
  Measurement of PI content of various tissue/cell extracts,Determination of PI concentration in biological fluids
• Sample Type
  Biological fluids (plasma, serum),Tissue and cell lysates
• Features Benefits
  This assay provides a convenient and non-ELISA alternative to detect cardiolipin amount in biological samples.,Validated (biological fluids, tissue/cell lysate),Sensitive, Specific
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Phosphoinositides are critical second messengers in a multitude of cellular processes. The most fundamental phosphoinositide, phosphatidylinositol (PI), can be phosphorylated at multiple sites around its inositol ring, leading to downstream activation. For instance, phosphatidylinositol 4-[mono]phosphate is involved in membrane trafficking, while cleavage of phosphatidylinositol triphosphate yields inositol triphosphate, a molecule involved in calcium signaling. Signaling is conveyed in many signaling proteins (including Protein Kinase B/Akt) via their domain referred to as a Pleckstrin homology, or PH domain, which is capable of binding to specific phosphoinositides. Biosynthesis of PI involves the action of phosphatidylinositol synthase on cytidine diacylglycerol and myo-inositol. The critical signal transducer enzymes, Phosphatidylinositolinositol-3-kinases phosphorylate PI at the 3 position hydroxyl of inositol, leading to modulation in pathways such as cell growth, survival, and motility. PI is typically at concentrations in the low- to mid-micromolar range in human serum and plasma, and is capable of activating second messengers such as Ca2+ and cyclic AMP. It is through these pathways that PI has been found to affect such biological events as the pro-inflammatory response and intestinal uptake. BioVision’s Phosphatidylinositol Assay Kit utilizes specific enzymes to generate an intermediate that then reacts with PicoProbeTM, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PI present in the sample. The assay is capable of detecting as little as 15 pmoles of phosphatidylinositol.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  PicoProbeTM   Phosphatidylinositol   Kit   Fluorometric  
• Short name
  PicoProbeTM Phosphatidylinositol Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbeTM Phosphatidylinositol test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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