TIP2;1 (delta), Delta-VM23

• Catalog number
  AS09 494
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-synthetic peptide conserved in Raphanus sativus TIP2;1 (delta) O82142
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  collection of antibodies to tonoplast proteins AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
• Molecular weight expected аpparent
  24.9 | 23 kDa
• Verified reactivity
  Raphanus sativus
• Possible reactivity
  Raphanus sativus
• No reactivity
  Arabidopsis thaliana, Pisum sativum
• Supplementary information
  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
• References
  to be added when available
• Scientific context
  TIPs belong to MIP/aquaporin protein family. Alternative name of radish TIP2;1 (delata) is delta-VM23.
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.42 kDa dimer is also detected by those antibodies.Protocol for isolation of plant vacuolar membranes can be found here.
• Protein number
  O82142
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  TIP2   delta   Delta-VM23  
• Short name
  TIP2 1 (delta), Delta-VM23
• Alternative name
  TIP2;1 (delta), Delta-VM23

Similar products