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Type
Conjugated Primary Antibody
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Conjugated with
Cy3
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
PSMG1
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Specificity
This antibody reacts specifically with PSMG1 Cy3
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
Cytoplasm
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Antigen Source
KLH conjugated synthetic peptide derived from human PSMG1
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Gene ID
8624
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
512,550nm/570,615nm
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Synonyms
Proteasome assembly chaperone 1; PAC-1; Chromosome 21 leucine-rich protein
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Also known as
PSMG1 Polyclonal Antibody Cy3
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Other name
Anti-PSMG1 Polyclonal Cy3
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-PSMG1 Cy3 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Polyclonal Antibodies antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
PSMG1
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Short name
Anti-PSMG1 Cy3
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
Cy3
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Alternative name
PSMG1 Polyclonal Antibody , Cy3 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
proteasome (prosome, macropain) assembly chaperone 1, C21LRP and DSCR2 and LRPC21 and PAC-1 and PAC1, PSMG1 and IDBG-3364 and ENSG00000183527 and 8624, proteasome binding, nuclei, Psmg1 and IDBG-179306 and ENSMUSG00000022913 and 56088, PSMG1 and IDBG-639136 and ENSBTAG00000013600 and 614817
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