-
Synonyms
CADM1; CADM-1; BL2; DKFZp686F1789, IGSF4; IGSF4A; NECL2; Necl-2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; sgIGSF; synCAM1, MGC149785, MGC51880
-
Alternative_names
CADM1; CADM-1; BL2; DKFZp686F1789, IGSF4; IGSF4A; NECL2; Necl-2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; sgIGSF; synCAM1, MGC149785, MGC51880
-
Description
May be involved in neuronal migration, axon growth, path finding, and fasciculation on the axons of differentiating neurons
-
Recombinant
Yes
-
Source
HEK293 cells
-
Purity by SDS PAGE
≥95%
-
Assay
SDS-PAGE
-
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rhCADM1 at 2 ug/ml (100 ul/well) can bind biotinylated human CRTAM with a linear range of 12.5 – 500 ng/ml.
-
Results
Measured by its binding ability in a functional ELISA. Immobilized rhCADM1 at 2 ug/ml (100 ul/well) can bind biotinylated human CRTAM with a linear range of 12.5 – 500 ng/ml.
-
Molecular Weight
This protein is fused with a polyhistidine tag at the C-terminus, and has a predicted molecular mass of 38.5 kDa. The predicted N-terminus is Gln 45. In DTT-reduced SDS-PAGE, rhCADM1 protein migrates as 38.5 kDa, 50 kDa and 55-85 kDa poly peptide due to different glycosylation.
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
-
Background Information
Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. Cell adhesion molecule 1 is a single-pass type I membrane protein which belongs to the nectin family. Cell Adhesion Molecule 1 contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain, and is also known as CADM1; BL2; DKFZp686F1789; IGSF4; IGSF4A; MGC149785; MGC51880; NECL2; Necl-2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; sgIGSF; synCAM1. CADM1 mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. CADM1 may be involved in neuronal migration, axon growth, path finding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
CADM1
-
Short name
CellExp™ CADM1, recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Humans
-
Alternative name
H. sapiens CellExp™ cellular adhesion molecule 1, H. sapiens Rec.
-
Alternative technique
rec
-
Alternative to gene target
cell adhesion molecule 1, BL2 and IGSF4 and IGSF4A and Necl-2 and NECL2 and RA175 and sgIGSF and ST17 and sTSLC-1 and SYNCAM and synCAM1 and TSLC1, CADM1 and IDBG-72227 and ENSG00000182985 and 23705, cell adhesion molecule binding, Cell surfaces, Cadm1 and IDBG-161668 and ENSMUSG00000032076 and 54725, CADM1 and IDBG-630721 and ENSBTAG00000000977 and 529873
-
