MSH2 Antibody, HRP Conjugated

• Catalog number
  bs-0758R-HRP
• Price
  Please ask
• Size
  0.1ml

• Long name
  MSH2 Polyclonal Antibody, HRP Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  HRP
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  MSH2
• Specificity
  This is a highly specific antibody against MSH2.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  80-130/935
• Subcellular location
  Nucleus
• Source
  KLH conjugated synthetic peptide derived from human MSH-2
• Gene ID number
  4436
• Swiss Prot
  P43246
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Additional conjugation
  HRP Conjugated
• French translation
  anticorps

• Gene target
  MSH2   Conjugated  
• Gene symbol
  MSH2-OT1, MSH2
• Short name
  Anti-MSH2
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  HRP
• Alternative name
  Anti-MSH2 PAb HRP
• Alternative technique
  antibodies
• Alternative to gene target
  mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli), COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, ADP binding, nuclei, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115

Gene info

• Identity
  HGNC:40319
• Gene
  MSH2-OT1
• Long gene name
  MSH2 overlapping transcript 1
• Locus
  2p16.3
• Discovery year
  2019-08-15
• Entrez gene record
  644093
• RefSeq identity
  • NR_120602
• Classification
  • Overlapping transcripts

Gene info

• Identity
  HGNC:7325
• Gene
  MSH2
• Long gene name
  mutS homolog 2
• Synonyms gene
  • COCA1
• Synonyms gene name
  • mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
  • mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Synonyms
  • HNPCC
  • HNPCC1
• Synonyms name
  • DNA mismatch repair protein Msh2
• GenBank acession
  • U03911
• Locus
  2p21-p16.3
• Discovery year
  1993-07-28
• Entrez gene record
  4436
• Pubmed identfication
  • 8484120
  • 9843200
• Classification
  • MutS homologs
• VEGA ID
  OTTHUMG00000128861
• Locus Specific Databases
  • Hereditary Non-Polyposis Colorectal Cancer, HNPCC
  • Global Variome shared LOVD
  • MSH2 database at LOVD-China
  • LOVD - Leiden Open Variation Database
  • LRG_218

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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