Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*
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Catalog number
22801
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Price
Please ask
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Size
100 Tests
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Description
Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* is used for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. It is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye.This kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.
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Shipping
Room Temperature
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Storage
Freezer
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Shelflife
6 months
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Chemical components
Component A: 200X JC-10 in DMSO ; Component B: Assay Buffer
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Excitation Wavelength
510 nm
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Emission Wavelength
525 nm
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Personal precautions
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*, please use the Cat. Nr.22801 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*
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Test
aat supplies other types of Assays as 1. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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Additional description
For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.Â
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Gene target
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Gene symbol
PIRC49, SNORD115-10, SNORD116-10, IGHV3OR16-10, IGKV2OR2-10, ERVK-10
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Short name
Meter JC-10 Mitochondrion Potential Assay Kit *Optimized for Assays*
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Technique
Flow Cytometry, Assay, Flow, assays
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Host
Assay
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Alternative name
cellular Meter JC-10 Mitochondrion Membrane Potential test reagent *Optimized to measure Flow Cytometry tests*
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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