Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* is used for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. It is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye.This kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.
Component A: 200X JC-10 in DMSO ; Component B: Assay Buffer
To order Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*, please use the Cat. Nr.22801 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*
aat supplies other types of Assays as 1.Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
For cells, cell lines and tissues in culture till half confluency.FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Meter JC-10 Mitochondrion Potential Assay Kit *Optimized for Assays*
Flow Cytometry, Assay, Flow
cellular Meter JC-10 Mitochondrion Membrane Potential test reagent *Optimized to measure Flow Cytometry tests*