This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: ApoE4
Alternative names of antibody target: Apolipoprotein E4, APOE 4, APOE4, APOE-4
Recommended usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human ApoE4 (Cat.# 4699-50) can be used as a positive control.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Recombinant human ApoE4
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer’s disease.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps