EZLys™ Bacterial Protein Extraction Reagent

  • Catalog number
    8001-500
  • Price
    Please ask
  • Size
    500 ml
  • Description
    A non-denaturing bacterial proteins and inclusion bodies extraction reagent.. A proprietary formulation prepared in a 50 mM Tris pH8.0 buffer and designed for gentle disruption of cell membrane to extract proteins. It provides a simple, rapid, low cost method for releasing expressed target protein for preparation, purification, or other applications. The EZLys™ reagent is an optically transparent, non-denaturing formulation which can be easily removed by dialysis. It is also useful for the preparation of high purity inclusion bodies for subsequent purification. This reagent does not interfere with cross linking and is suitable for use in the BCA or Bradford protein assays. Ideal pH range is pH 5 -10. The 100 ml and 500 ml reagent are sufficient for protein extraction from 25 g and 125 g of cell paste.
  • Product Highlights
    • Kit features- 1) Complete cell lysis without mechanical stress which is tedious and can damage proteins. 2) The extracted proteins are in native, non-denatured form. 3) The reagent does not interfere with down-stream functional testing or protein assays. 4) The reagent can easily be removed by dialysis or spin-column buffer exchange.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
MeSH Data
  • Name
  • Concept
    Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
  • Tree numbers
    • E01.370.225.812.735.830
    • E05.200.812.735.830
    • E05.478.566.380.810
    • E05.478.566.639.810
    • E05.478.594.760.830
    • E05.601.470.380.810
    • E05.601.470.639.810
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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