Recombinant Human Platelet-Derived Growth Factor AA/PDGF-AA (N-6His)
-
Catalog numberCH46-50
-
PricePlease ask
-
Size50 ug
-
-
DescriptionRecombinant Human Platelet-derived growth factor AA is produced by our E.coli expression system and the target gene encoding Ser87-Thr211 is expressed with a 6His tag at the N-terminus.
-
Species reactivityHuman
-
OriginEscherichia coli
-
Peptide sequenceMNHKVHHHHHHMSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT
-
Estimated molecular weight15,9 kDa
-
Protein purityGreater than 95% as determined by reducing SDS-PAGE.
-
Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
-
Shipping conditionAmbient/Room Temperature
-
Package formLyophilized from a 0.2 µm filtered solution of PBS,pH7.4.
-
Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
-
Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
-
UniProt numberP04085
-
Additional descriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
SourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolPDGFA, TEAD1
-
Short nameRecombinant Platelet-Derived Growth Factor AA/PDGF-AA (N-6His)
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHuman, Humans
-
Alternative nameHuman PDGF-AA(N-6His)
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene nameplatelet derived growth factor subunit A
-
Synonyms gene name
- platelet-derived growth factor alpha polypeptide
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameTEA domain transcription factor 1
-
Synonyms gene
-
Synonyms gene name
- atrophia areata, peripapillary chorioretinal degeneration
- TEA domain family member 1 (SV40 transcriptional enhancer factor)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1994-09-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- TEA domain transcription factors
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data