CF633 monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
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Catalog number
20212
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Price
Please ask
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Size
0,25 mL
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Description
CF633 monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
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Category
CF Dye Antibodies
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Shipping temperature
15°C to 30°C
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Shipping conditions
n/a
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Storage
-30°C to -2°C
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Hazard pictogram
GHS07 exclamation mark, GHS08 systemic health hazards
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Signal word
Warning, Danger
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Hazard statements
H315 Causes skin irritation H319 Causes serious eye irritation H335 May cause respiratory irritation H373 Causes damage to organs through prolonged or repeated exposure category 2 ( lungs) H372 Causes damage to organs through prolonged or repeated exposure category 1 (gastrointestinal) (oral)
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This Biotium Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biotium FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Short name
CF633 monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/
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Technique
anti-, Mouse, anti, FITC, IgG, antibody to, antibodies, Fluorescein, IgGs, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG, IgG
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
CF633 monoclonal mouse Antibody tofluorescein (fluorecein) Immunoglobulin G, 2 mg/milliliter
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Alternative technique
murine, antibodies, fluorescine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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