p53 (phospho-S6) polyclonal antibody
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Catalog number
BS4148-100ul
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Price
Please ask
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Size
100ul
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Alternative Name
Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
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Immunogen
Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 6.
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Swiss Prot
P04637
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Reactivity
Human,Mouse,Rat
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Applications
WB IHC IP
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Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
p-p53 (S6) polyclonal antibody detects endogenous levels of p53 protein when phosphorylated at Ser6.
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Usage
Western blot (WB) analysis of p-p53 (S6) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with Etoposide(20ng/ml) for 5 minutes whole cell lysate Lane3:HEK293T treated with Etoposide(20ng/ml) for 30 minutes whole cell lysate Lane4:HEK293T treated with Etoposide(20ng/ml) for 2 hours whole cell lysate
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BiowMW
~53,43 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
RPS6, MRPS6, PSMC4
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Short name
p53 (phospho-S6) polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
p53 (phosphorilated-S6) polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene name
ribosomal protein S6
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Synonyms
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Synonyms name
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Locus
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Discovery year
1990-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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