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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318)
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Antibody s target
Human AKT1 (pTyr315)
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Antibody s full description
Rabbit Anti-Human AKT1 (pTyr315) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 55.686 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Cell Membrane
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Tissue specificity
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
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Scientific context
AKT1 is a protein-serine/threonine kinase which is inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, AKT1 is recruited to the cell membrane and phosphorylated to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. AKT1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. Upregulation of the PI3K/AKT pathways has been implicated in a variety of cancers.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_005154
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Gene number
207
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Protein number
P31749
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
AKT1
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Short name
Rabbit Anti- AKT1 (pTyr315) Polyclonal: ATTO 633
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 633
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman v-akt murine thymoma viral oncogene homolog 1 (pTyr315) polyclonal: ATTO 633
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
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