HAMSTER ANTI MOUSE CD27:Biotin Antibody

  • Catalog number
    GEN224636
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    CD27
  • Other names
    CD27 antigen; CD27 molecule; CD27 antigen; CD antigen 27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    CD27
  • Gene name synonims
    N/A
  • Other gene names
    Cd27; Cd27; Tnfrsf7; MGC112688
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG
  • Clone
    LG.3A10
  • Host organism
    Hamster
  • Species reactivity
    Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    CD27 Clone LG.3A10 recognises mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a costimulatory signal in lymphocyte proliferation and immunoglobulin production. CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein). ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Biotin (Purified IgG conjugated to Biotin - liquid)
  • Concentration
    IgG concentration 500ug/1000ul
  • Storage and shipping
    Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE. the antibody should be stored undiluted. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Immunoprecipitation (IP), Immunohistology - Frozen (IHC), Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
  • Conjugation
    Biotinylated
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
    CD27  
  • Gene symbol
    CD27-AS1, CD27
  • Short name
    ANTI MOUSE CD27: Antibody
  • Technique
    anti mouse, Antibody, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
  • Host
    Hamster
  • Label
    Biotin
  • Species
    Mouse, Hamsters, Mouses
  • Alternative name
    Anti-HAMSTER ANTI MOUSE CD27:Biotin
  • Alternative technique
    antibodies, murine
  • Alternative to gene target
    CD27 molecule, S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55, CD27 and IDBG-14014 and ENSG00000139193 and 939, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, Extracellular, Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940, CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    CD27 molecule
  • Synonyms gene
  • Synonyms gene name
    • tumor necrosis factor receptor superfamily, member 7
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1992-06-10
  • Entrez gene record
    939
  • Pubmed identfication
  • Classification
    • Tumor necrosis factor receptor superfamily
    • CD molecules
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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