-
Products_type
Secondary Antibody
-
Products_short_name
[IgG Fc]
-
Products_name_syn
[Goat anti-Mouse IgG Fc - Affinity Pure]
-
Reactivity
Mouse. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin mouse serum proteins light chains on all mouse immunoglobulins mouse IgG, F(ab)2 fragment serum proteins from bovine, horse, or human
-
Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG
-
Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Mouse IgG.
-
Form
Clear, colorless liquid
-
Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
-
Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
-
Tested_application
ELISA (EIA), Immunohistochemistry (IHC)
-
Test
A high affinity purification column was use to purify Goat anti-Mouse IgG Fc - Pure, Conjugate, min x w/ , serum proteins by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Mouse IgG Fc - Pure, Conjugate, min x w/ , serum proteins supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Biotinylated
-
Latin name
Capra aegagrus hircus, Mus musculus
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
-
Group
Sera
-
Gene target
-
Short name
Goat anti-Mouse IgG Fc - Pure, Conjugate, min x w/ , serum proteins
-
Technique
affinity, anti-, Mouse, serum, anti, Goat, Pure, IgG, FC, antibody to, antibodies, goats, IgGs, mouses, pure products
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
IgG, IgG
-
Label
Biotin
-
Species
Human, Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Humans, Mouses
-
Alternative name
caprine Antibody toMouse Immunoglobulin G fragment c - protein Pure, biotinilated Conjugate, min x w/bovine, horse and H. sapiens blood serum proteins
-
Alternative technique
sera, murine, antibodies, purified, caprine, immunoglobulins
-
Tissue
serum
-
