anti-PDGF-BB Antibody
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Catalog numberXP-5255Bt
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PricePlease ask
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Size0.05 mg
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Gene namesSIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
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Antibodie s labelBiotin
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PurityAnti-hPDGF-BB specific antibody was purified by affinity chromatography and then biotinylated.
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Ab raised inRabbit
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TypePolyclonal
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AntigenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor-BB).
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Immuno applicationELISA, Western Blot
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Antibody adviceELISA:To detect hPDGF-BB by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich:To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human PDGF-BB (XP-5255) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.Western Blot:To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep atPDGF-BB antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works withHuman
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Omim nr129724
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Ncbi nrP01127
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Vial with antibodyLyophilized
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NCBI GENE nr5155
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GENE symbolPDGFB
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Antigene nameplatelet-derived growth factor beta polypeptide
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SourceHomo sapiens
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Protein nrP01127
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPDGFA
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Short nameanti-PDGF-BB Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toPDGF-BB (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameplatelet derived growth factor subunit A
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Synonyms gene name
- platelet-derived growth factor alpha polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data