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Target antigen
Spectrinalpha and beta
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Clonality
Monoclonal antibody
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Clone
SB-SP1
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human
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Analyses
WB
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Immunogen
Human erythrocyte spectrin.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the Spectrin(alpha and beta) Antibody ( SBSP1)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Spectrin(alpha and beta) Antibody ( SBSP1) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.
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Related articles
1. Watkins, P. C.; Eddy, R.; Forget, B. G.; Chang, J. G.; Rochelle, R.; Shows, T. B. : Assignment of a non-erythroid spectrin gene to human chromosome 2. (Abstract) Am. J. Hum. Genet. 43: A161, 1988. 2. Ikeda, Y.; Dick, K. A.; Weatherspoon, M. R.; Gincel, D.; Armbrust, K. R.; Dalton, J. C.; Stevanin, G.; Durr, A.; Zuhlke, C.; Burk, K.; Clark, H. B.; Brice, A.; Rothstein, J. D.; Schut, L. J.; Day, J. W.; Ranum, L. P. W. : Spectrin mutations cause spinocerebellar ataxia type 5. Nature Genet. 38: 184-190, 2006.
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Gene Name
SPTA1; SPTB
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Protein Name
Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic
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Gene Full Name
spectrin, alpha, erythrocytic 1(elliptocytosis 2); spectrin, beta, erythrocytic
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Synonyms
EL2|HPP|HS3|SPH3|SPTA|EL3|HS2|SPH2|HSPTB1|Erythroid alpha-spectrin
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Uniprot ID
Human(P02549), Mouse(P08032), Rat(D4A678), Human(P11277), Mouse(P15508), Rat(Q6XDA0)
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Entrez GeneID
Human(6708), Mouse(20739), Rat(289257), Human(6710), Mouse(20741), Rat(314251)
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Description
The Spectrin(alpha beta) Antibody ( SBSP1) is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
