Mouse Monoclonal IgM Anti-human CD15 [MMA/LeuM1]
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Catalog numberCD15-1
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PricePlease ask
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenU937 histiocytic cell line
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SpecificityHuman CD15 (3-fucosyl-N-acetyllactosamine)
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Species reactivityHuman
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgM
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Antibody s cloneMMA/LeuM1
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Product is supplied asliquid concentrate
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Recommended concentration for use1:15-1:25
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Protein concentration50 µg/ml
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Positive controlReed-Sternberg cells in Hodgkin's disease
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PretreatmentFormaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solution pH 6 (Art. No. DE000) at 96-100°C followed by short term treatment with Pepsin (0.1%)
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Tested applicationsIHC(P), FACS
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Recommended secondary reagentAs secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.
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Storage methodStore at 2-8°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Skubitz K. et al. In: White Cell Differentiation Antigens. New York / Oxford University Press 1989; 800-805.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Testbio logo supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolMMD, DSCR8, FUT4
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Short nameResearch
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TechniqueMouse monoclonal, anti-Human, anti-, Mouse, anti, IgM, antibody to, antibodies, antibodies against human proteins, IgMs, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgM, IgM
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SpeciesHuman, Humans, Mouses
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Alternative nameCD15, human
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Alternative techniquemurine, antibodies, immunoglobulin-m
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Gene info
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Identity
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Gene
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Long gene namemonocyte to macrophage differentiation associated
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Synonyms gene name
- monocyte to macrophage differentiation-associated
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-07
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Entrez gene record
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Pubmed identfication
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Classification
- Progestin and adipoQ receptor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDown syndrome critical region 8
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Synonyms gene
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Synonyms gene name
- chromosome 21 open reading frame 65
- Down syndrome critical region 8 (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-09-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 4
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Synonyms gene
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Synonyms gene name
- ELAM ligand fucosyltransferase
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Fucosyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data