Purified rabbit skeletal muscle actin
Alpha-Actin sarcomeric (a-sr-1) specific for skeletal- and heart-muscle
Human, amphibia, bony fish, cattle, guinea pig, reptiles, rabbit, rat, sheep
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
Product is supplied as
ready to use
Recommended concentration for use
No protease pre-treatment necessary. Blocking of endogenous peroxidase and alkaline phosphatase recommended depending on the detection system in use.
1. Skalli O, Gabbiani G, Babai F, Seemayer TA, Pizzolato G, Schurch W. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am. J. Pathol., 130; 515ff (1988) 2. Babai F, Skalli O, Schurch W, Seemayer TA, Gabbiani G. Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms.Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(5):263-77. 3. Hasegawa T, Matsuno Y, Niki T, Hirohashi S, Shimoda T, Takayama J, Watanabe C, Kaneko A, Sano T, Sato M, Suzuki J. Second primary rhabdomyosarcomas in patients with bilateral retinoblastoma: a clinicopathologic and immunohistochemical study. Am J Surg Pathol. 1998 Nov;22(11):1351-60. 4. Eusebi V, Damiani S, Pasquinelli G, Lorenzini P, Reuter VE, Rosai J. Small cell neuroendocrine carcinoma with skeletal muscle differentiation: report of three cases. Am J Surg Pathol. 2000 Feb;24(2):223-30. Review. 5. Tornoczky T, Kalman E, Sapi Z, Orosz Z, Pajor L. Cytogenetic abnormalities of alveolar soft-part sarcomas using interphase fluorescent in situ hybridization: trisomy for chromosome 7 and monosomy for chromosomes 8 and 18 seem to be characteristic of the tumor. Virchows Arch. 2001 Feb;438(2):173-80.
The Mouse Monoclonal IgM Anti-Alpha-sarcomeric Actin [5C5] is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.