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Perchlorate Free Assay Kit

Perchlorate Free Assay Kit is available 2 times from Biovision labs

K226-100 | Perchlorate-Free Hydroxyproline Assay Kit size: 100 assays | 640.24 USD

Catalog number K226-100
Supplier Biovision
Price 640.24 USD
Size 100 assays
Description Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich’s reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is both toxic and highly reactive, requiring special handling and waste-disposal protocols. BioVision’s Hydroxyproline Assay Kit II (Perchlorate-Free) uses a proprietary acidic developer solution to accurately measure Hyp in tissue and protein/peptidase hydrolysates without the use of hazardous perchlorates. It is a quick and convenient protocol, yielding a brightly-colored chromophore with an absorbance peak at 560 nm. The assay can detect a minimum of 0.05 µg hydroxyproline per well in a 96-well format and generates colorimetric results that are virtually identical to the classical perchloric acid-based method.
Highlights Convenient and easy to use kit with vert simple procedure because it takes less than ~60 minutes. Faster than Perchlorate-Based Assay, PCA-free
Kit reactivity Mammalian
Kit is besf for Estimation of hydroxyproline concentration in various biological samples
Other name Hydroxyproline Assay Kit
Detection procedure Absorbance (OD = 560 nm).
Kit contents The Perchlorate-Free Hydroxyproline Assay Kit contains Oxidation Buffer, Chloramine T Concentrate, Developer Solution, DMAB Concentrate (in DMSO), Hydroxyproline Standard (1 mg/ml) and a Microplate Sealing Film
Samples Animal tissues protein/peptide hydrolysates serum urine
Assay type Hydroxyproline
Test Biovision supplies other types of Assays as 1.
Gene targetPerchlorate-Free Hydroxyproline Kit
Short name Perchlorate-Free Hydroxyproline Assay Kit
Alternative name Perchlorate-Free Hydroxyproline test reagent
MeSH Data
Name Hydroxyproline
Tree numbers
  • D12.125.072.401.623.478
Product images
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K406-100 | Perchlorate-Free Total Collagen Assay Kit size: 100 assays | 640.24 USD

Storage Store this kit at -20°C for 12 months
Delivery This product is shipped with gel pack
Datasheet Please contact us to inquire the datasheet and an example certificate of analysis
Released This assay kit is on the market since May 2017
Technique Assay
Host Assay
Alternative technique arrays
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue total
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Perchlorate-Free Hydroxyproline Assay Kit -
+32-(0)1-658-90-45 [email protected]
  • Perchlorate-Free
  • Hydroxyproline
  • Kit
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